Journal: Human reproduction (Oxford, England)

Article Title: Engraftment potential of human placenta-derived mesenchymal stem cells after in utero transplantation in rats.

doi: 10.1093/humrep/den356

Figure Lengend Snippet: Figure 3 Hematoxylin-eosin (HE) stain and immunofluorescence of fetal rat organs at embryonic day (E)21, 5 days after transplantation of bisbenzimide-labeled hPMCs. hPMCs (blue fluorescence) were present in various fetal rat tissues. Rat tissues were immunostained using fluorescein isothiocyanate (FITC)-conjugated antibodies. (A) HE stain of rat heart. Inset area: a serial section was immunostained for troponin I and was shown in separate channels (B–D) with insets (2) showing costaining in individual cells arrowed. (E) HE stain of rat lung. Inset area: a serial section was immunostained for surfactant protein D and imaged using a laser-scanning confocal microscope (F). The panels to the right and below are y-z and x-z projections showing surfactant protein D in cytoplasmic areas adjacent to labeled cell nuclei. (G) A negative control of rat lung was immunostained for non-specific mouse immunoglobulin G. (H–J) Further immunofluorescence images indicating co-localization of the monoclonal antibody specific to surfactant protein D with hPMCs. (K) Several blue-stained hPMC nuclei in an area of rat lung. Some are clearly Ki67-positive (FITC-staining; arrow). (L) HE stain of rat liver. Inset area: a serial section was immunostained for human albumin and was shown in separate channels (M–O) with insets (2) showing costaining in individual cells arrowed. (P) HE stain of rat spleen. Inset area: a serial section was immunostained for CD45 to show the spleen structure. Immunofluorescence is shown in separate channels (Q–S) with insets (2) showing the hPMC (arrowed) in rat spleen. (T) HE stain of rat placenta. Inset area: a serial section was immunostained for cytokeratin 8 and 18. Immunofluorescence is shown in separate channels (U–W). Numerous hPMCs are seen in the placental labyrinth (W). Scale bar: 30 mm.

Article Snippet: The cell surface phenotype of hPMCs was characterized using a panel of phycoerythrin (PE)- or fluorescein isothiocyanate (FITC)-conjugated antibodies purchased from Serotec (Oxford, UK), Chemicon (Temecula, CA, USA), or BD Biosciences-Pharmingen (NJ, USA) using standard fluorescence-activated cell sorting analysis.

Techniques: H&E Stain, Transplantation Assay, Labeling, Microscopy, Negative Control, Staining

Journal: Human reproduction (Oxford, England)

Article Title: Engraftment potential of human placenta-derived mesenchymal stem cells after in utero transplantation in rats.

doi: 10.1093/humrep/den356

Figure Lengend Snippet: Figure 5 Flow cytometric analysis of HLA-ABC-positive hPMCs in the rat peripheral blood and bone marrow after in utero transplantation of hPMCs. Representative data showing percentages of human HLA-ABC-positive hPMCs in peripheral blood (A) or bone marrow (B) of recipient rat at E21 and 3 weeks after birth. Control: rat without hPMC transplantation. The upper panels of (A) and (B) show the gating strategy in forward versus side scatter. Dual color flow cytometric scatter plot of mononuclear cells from peripheral blood (C) or bone marrow (D) from a hematopoietic chimera stained with anti-CD45-FITC and anti-HLA- ABC-phycoerythrin antibodies. The cells were counted by 2-color flow cytome- try. Representative analysis of the hematopoietic lineage reconstitution of rat transplanted with hPMCs (analyses were conducted at E21 and 3 weeks post-natally). Dot-plots illustrate the contribution of hPMC to the hematopoietic lineage (CD45) in a representative animal.

Article Snippet: The cell surface phenotype of hPMCs was characterized using a panel of phycoerythrin (PE)- or fluorescein isothiocyanate (FITC)-conjugated antibodies purchased from Serotec (Oxford, UK), Chemicon (Temecula, CA, USA), or BD Biosciences-Pharmingen (NJ, USA) using standard fluorescence-activated cell sorting analysis.

Techniques: In Utero, Transplantation Assay, Control, Staining